Assistant Professor

Ph.D. National Sun Yet-sen University, 2001

Neuroscience; Molecular biology;

E-mail: wanglh@nmmba.gov.tw; wanglh@mail.ndhu.edu.tw

Tel: 886-8-8825001#1366

Fax: 886-8-8825041

• Research Interest
  In order to investigate related genes that involve in the cell growth and differentiation, we established a tip of coral, Seriatopora hystrix cDNA library and analyzed their transcriptomes (or Expressed Sequence Tags, ESTs). Among 750 cDNA clones that were picked and subjected to sequencing, 740 were readable and have been annotated by way of bioinformatics technology. In brief summary, a total of 351 ESTs were identified to be orthologous to 280, unique known proteins in LocusLink database (NCBI, USA), 37 ESTs were identified to be matched (E<0.0001) with sequences in dbEST and 352 ESTs were not identifiable.
• Publications
  1. Tsai, C.L. and Wang, L.H., 1997, Effects of estradiol on the serotonin secretion and turnover in the hypothalamus of male tilapia, Oreochromis mossambicus. Gen. Comp. Endocrinol., 106:175-180. (SCI)
  2. Tsai, C.L. and Wang, L.H., 1997, Effects of thermal acclimation on the neurotransmitters, serotonin and norepinephrine in the discrete brain of male and female tilapia, Oreochromis mossambicus. Neurosci. Lett., 233:77-80. (SCI)
  3. Tsai, C.L. and Wang, L.H., 1997, Effects of estradiol and testosterone on the serotonin content and turnover in the brain of tilapia embryo. Biogenic Amines, 13:19-28. (SCI)
  4. Tsai, C.L. and Wang, L.H., 1997, Effects of androgen replacement on the serotonin and norepinephrine content in the brain of male tilapia, Oreochromis mossambicus. Biogenic Amines, 13:109-122. (SCI)
  5. Tsai, C.L. and Wang, L.H., 1998, Effects of gonadal steroids on the noradrenergic activity in the early developing tilapia brain. Biogenic Amines, 14:591-598 (SCI)
  6. Tsai, C.L., Wang, L.H. and Jou, I.M., 1998, Effects of temperature and photoperiod on the early development of central nervous system. FASEB J., 12:1854-1854 (SCI)
  7. Wang, L.H. and Tsai, C.L., 1999, Effects of gonadal steroids on the GABA and glutamate contents of the early developing tilapia brain. Dev. Brain Res., 114:273-276. (SCI) 
  8.  Tsai, C.L. and Wang, L.H., 1999, Effects of gonadal steroids on the serotonin synthesis and metabolism in the early developing tilapia brain. Neurosci. Lett., 264:45-48. (SCI)
  9. Tsai, C.L. and Wang, L.H., 2000, Sexual difference in the responses of serum calcium concentration to temperature and estrogen in tilapia, Oreochromis mossambicus. Zool. Studies, 39:55-60. (SCI)
  10. Tsai, C.L., Wang, L.H., and Wen, Z.H., 2000, Alteration of norepinephrine and serotonin contents in the discrete brain of male and female tilapia, Oreochromis mossambicus, during the lower temperature acclimation. Biogenic Amines, 15: 655-667. (SCI)
  11. Wang, L.H. and Tsai, C.L., 2000, Effects of temperature on the deformity and sex differentiation of tilapia, Oreochromis mossambicus. J. Exp. Zool., 286:534-537. (SCI)
  12. Wang, L.H. and Tsai, C.L., 2000, Temperature affects the development of the central nervous system in tilapia, Oreochromis mossambicus. Neursci. Lett., 285:95-98. (SCI) 
  13.  Tsai, C.L. and Wang, L.H., 2000, The alteration of GABA and glutamate contents in the discrete brain of female tilapia during certain stages of gonadal cycle. Neurosci. Lett., 291:171-174 (SCI)
  14. Tsai, C.L., Wang, L.H., Chang, C.F. and Kao, C.C., 2000, Effects of gonadal steroids on brain serotonergic and aromatase activity during the critical period of sexual differentiation in tilapia, Oreochromis mossambicus. J. Neuroendocrinol., 12:894-898. (SCI)
  15. Tsai, C.L., Wang, L.H. and Lin, Y.H., 2001, Effects of estrogen and neurotransmitters on the primary cultures of tilapia brain from the different ages. Dev. Brain Res.,129:111-113. (SCI) 
  16.  Tsai, C.L., Wang, L.H. and Fang, L.S., 2001, Estradiol and para-Chlorophenylalanine down-regulate the expression of brain aromatase and estrogen receptorα mRNA during the critical period of feminization in tilapia, Oreochromis mossambicus. Neuroendocrinology, 74:325-334. (SCI)
  17. Tsai, C.L., Wang, L.H. and Tsai, C.C., 2002, Role of serotonin, γ-aminobutyric acid, and glutamate in the behavioral thermoregulation of female tilapia during the prespawning phase. J. Exp. Zool., 293:443-449. (SCI)
  18. Tsai, C.L., Chang, S.L., Wang, L.H. and Chao, T.Y., 2003, Temperature influences the ontogenetic expression of aromatase and estrogen receptor mRNA in the developing tilapia brain. J. Neuroendocrinol., 15:97-102. (SCI)
  19. Huang Y.S., Wang, L.H., and Tsai, C.L., 2004, Photoperiod affects the development of central neurotransmitter systems of tilapia, Oreochromis mossambicus. Neurosci. Lett. 355:201-204. (SCI)
  20. Shiue Y.L., Wang, L.H., Chao, T.Y., Lin, C.C. and Tsai, C.L., 2004, EST-based identification of genes expressed in the hypothalamus of adult tilapia, Oreochromis mossambicus. Biochem. Biophys. Res. Comm. 316:523-527. (SCI)
  21. Chen, M.C., Cheng, I.M., Wang, L.H., Lin, C.C., Huang C.Y., Liu, M.C., P.J. Sung, and Fang, L.S. 2004, Cloning and characterization of the first cnidarian ADP-ribosylation factor, and its involvement in the aiptasia-symbiodinum endosymbiosis. Mar. Biotechnol. 6:138-147. (SCI)
  22. Wang, L.H. and Tsai, C.L., 2004, Effects of photoperiod on the development of central glutamate system in tilapia, Oreochromis mossambicus. Dev. Brain Res. 152:79-82. (SCI)
  23. Wang, L.H. and Tsai, C.L., 2005, Photoperiod influences the ontogenetic expression of aromatase and estrogen receptor a in the developing tilapia brain. Gen. Comp. Endocrinol. (in press). (SCI)
  24. Wang, L.H. and Tsai, C.L., 2006, Influence of Temperature and Gonadal Steroids on the Ontogenetic Expression of Brain Serotonin 1A and 1D Receptors During the Critical Period of Sexual Differentiation in Tilapia, Oreochromis mossambicus. Comp. Biochem. Physiol. B (in press). (SCI)